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HI3D

Site 1 (click me) | Site 2 (click me)

HI3D is a Web app allowing users to index the helical parameters (twist, rise, csym) of a 3D density map (asymmetric reconstructions, sub-tomogram averages, and helical reconstructions). It generates a cylindrical projection of the 3D map, computes the autocorrelation function of the cylindrical projection, and then uses two distinct lattice identification methods to obtain a consistent solution of the helical parameters.

Usage

  • No software installation is needed. Just click one of the above App sites to index your structure!
  • If the Web apps have issues (mostly from limited resources offered by the free hosting service), you can run a local Web app on your computer:
    • Install helicon
    • Run command: helicon hi3d

Gallery

Open Source/Development

The source code and all revisions are available on GitHub

Citation 

If you have found HI3D useful for your project, please cite:

Chen Sun, Brenda Gonzalez, Wen Jiang. Helical indexing in real space. Scientific Reports, 12(1), 1–11. https://doi.org/10.1038/s41598-022-11382-7